The preliminary study on the quantitative color image analysis on immunohistochemical staining of squamous cell lung carcinoma

Abstract

To study the differences and usage of the color indexes in the quantitative image analysis on immunohistochemical staining of squamous cell lung carcinoma between moderate differentiation and poor differentiation. S-P method was used in p53, PCNA and p16 immunohistochemical staining of squamous cell lung carcinoma. Quantitative image analysis was performed with Computer Assisted Pathological Image Analysis System. There was no well differentiation case of the 20 cases. Positive stain of p53 was observed in 6 cases in moderate differentiation group and 6 cases in poor differentiation group respectively. Positive PCNA stain existed in 7 cases in moderate differentiation group and 6 cases in poor differentiation group respectively. Five cases in moderate differentiation group and 6 cases in poor differentiation group had p16 expression deletion. The saturation of p53 staining was lower in moderate differentiation group (4.95%) than that in poor differentiation group (10.95%) (t=4.208,P=0.002). The saturation of PCNA staining was lower in moderate differentiation group (7.74%) than that in poor differentiation group (12.98%) (t=2.416,P=0.034). The hue of p16 staining was lower in moderate differentiation group (53.98⁰) than that in poor differentiation group (62.32⁰) (t=2.784,P=0.021). The color indexes in the quantitative immunohistochemical image analysis may reflect the differentiation degree of squamous cell lung carcinoma.