The Preliminary Study on Safety of Using Mangosteen Peel Extract as Natural Herbs

Abstract

Background: The properties of mangosteen skin as anti-inflammatory have been described by many researchers both in vitro and in vivo. Over the past few years, mangosteen peel extract is used as a natural herbal remedy either orally or topically. However, the potential toxicity of extracts and formulations containing mangosteen peels is still a matter of little concern. Objective: To prove that mangosteen skin extract is safe to use as natural herbal medicine. Methods: Experiments with laboratory tests of microbiological tests and acute toxicity tests with samples of 30 Balb / C mice aged 8-10 weeks weight 25-35 g, divided into 6 groups in which 5 groups of intervention (giving mangosteen skin extract) with different dose (5, 50, 300, 2000 and 5000 mg / kgBB) and 1 control group. Parameters observed included weight, motor activity, salivation, respiration, defecation, urination, piloerection every day for 14 days while the observed organ was liver. Behavioral data and organ test results were used to evaluate the toxic effects of mangosteen skin extract. Data was analyzed with ANOVA and Post Hoc Test. Results: Most subjects' mean body weight from day 0 to day 14 tended to stabilize with a slight increase or decrease of less than 1%. The results of observation for 14 days after the intervention to each group there was no death and no effect on the motor system, saliva, respiration, defecation, urination and piloerection and did not happen itch allergies. Based on Post Hoc Test (LSD), there was no significant difference in mean liver weight group with highest dose (5000 mg / kgBB) and control group. Conclusion: Extract of mangosteen skin with dosage ≤ 5000 mg/kg BW is not toxic and safe to use as natural herbal medicine.