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Abstract
Immobilized enzyme reactors (IMERs) are a powerful and essential part of multidimensional liquid chromatography–tandem mass spectrometry (MDLC–MS/MS) approaches that enable online identification, characterization, and quantification of post-translational modifications of therapeutic antibodies. This review gives an overview of commercially available and selected trypsin IMERs in regard to their application in LC-based and automated sample preparation. Additionally, we address the challenges of IMER application in online systems and the advantages of self-made IMERs.
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