The potential use of fish collagen as a new functional materials due to its good immune-compatibility

Abstract

As a marine-derived biological macromolecule, fish collagen is attracting more and more attention for its potential application in the biomedical field. Currently, there is no systematic evaluation of immuno-compatibility of fish collagen in vivo. In the present study, 200 mg/kg hydrolyzed tilapia fish collagen (HTC) was injected subcutaneously into mice for 21 consecutive days. To evaluate the effects of HTC on spleen lymphocytes proliferation, cell counting assay (CCK-8) was performed. Flow cytometry was applied to study the effect of HTC on the apoptosis of spleen lymphocytes. Mouse serum immunoglobulin G (IgG) and immunoglobulin M (IgM) were determined by Enzyme-linked immunosorbent assay (ELISA). The results showed no significant difference for the proliferation and apoptosis of spleen lymphocytes between the HTC group and the control group (subcutaneously injected with 0.9% NaCl for 21 consecutive days). And there was no significant difference for serum IgG and IgM levels between the control group and HTC group. The above results showed that the hydrolyzed tilapia fish collagen possesses good immune-compatibility, which can potentially be used as biocompatible implanted biomaterials in vivo.