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Abstract
AbstractThe potential of an interface for the on‐line coupling of microcolumn liquid chromatography (LC)and a flame photometric detector (FPD) has been further investigated. With the micro‐LC/FPD system, relatively high‐molecular‐weight polar compounds such as cyclic adenosine monophosphate, guanosine monophos‐ phate, glucose monophosphate, fructose monophosphate, and phytic acid were separated and selectively detected. In order to increase the sensitivity, on‐line preconcentration with a microprecolumn inserted in the rotorof a Valcovalve has been applied. Preliminary results have shown that an injection volume of at least 500 1‐11 water containing organophosphorus acids at a 5–50 ng/rnl concentration level is possible.
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