The potential of Moringa oleifera extract as a phytobiotic candidate for the growth of Lactobacillus fermentum bacteria by in vitro

Abstract

Moringa oleifera is used to explore the biodiversity of the ability of herbal plants as a candidate phytobiotic. The total carbohydrate content in the ethanol extract is 42% consisting of monosaccharides, disaccharides, and oligosaccharides which are gut micro-flora nutrient. The study aimed to discover the effect of moringa leaf extracts with different dosages to improve Lactobacillus fermentum bacteria’s growth. The study employed moringa leaf extracts and Lactobacillus fermentum bacteria then divided them into four groups, i.e., control group (P0), treatment P1 with moringa leaf extracts of 0.1% dosage, treatment P2 with moringa leaf extracts of 0.2%, and treatment P3 with moringa leaf extracts of 0.3% dosage. At the end of the treatment, each colony was calculated using the Total Plate Count method. Data obtained were analyzed using ANOVA and subjected to the Duncan test. The study results show that moringa leaf extracts with dosages of 0.1%, 0.2%, and 0.3% could improve Lactobacillus fermentum bacteria’s growth. The average growth log of Lactobacillus fermentum bacteria on treatment P0 was 3,532 log CFU/ml, 4,320 log CFU/ml for treatment P1, 4,368 log CFU/ml for treatment P2, and 4,480 log CFU/ml for treatment P3 as the highest value.