Abstract
Silver nanoparticles had been synthesized through the bioreduction process using daruju (Acanthus ilicifolius) leaf extract as a bioreductant. The variation of the formula used is a mixture of daruju leaf extract with a 0.01 M AgNO3 solution with volume ratios of 2:1, 1:1, 1:2, and 1:3. The research results show that UV-Visible spectrophotometry confirmed that a volume ratio of 1:1 is the optimum formula with an absorption value of 2.346 at the maximum wavelength 440 nm. Synthesis of silver nanoparticles with daruju leaf extract has a strong inhibition zone about 10.42 mm. Proving that the synthesis of silver nanoparticles with extract daruju leaf can be used as an antibacterial agent against E.coli bacteria.
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