Abstract
This study aims to find the most appropriate indigenous bacteria as Naphthalene and Phenanthrene degrading agents carried out in the growth response test. This research is very important to find the best bacterial agent, which in the future can reduce the impacts of oil pollution, as well as from the presence of oil sludgeBacterial cultures added in the media as much as 5% (v / v), which had previously been measured OD660nm = 0.5. The growth response test was carried out by growing 20 mL of bacteria in AMS media which was added with PAH substrate, naphthalene or Phenanthrene in various concentrations: 200 ppm, 500 ppm, 800 ppm, and 1000 ppm. In the naphthalene substrate with 24-hour incubation, the best treatment was in isolates A with a concentration of 200 ppm. In the phenanthrene substrate 24 hours incubation, the best treatment was in isolate A at 200 ppm, and at 48 hours incubation. Bacterial isolates F in 24-hour incubation increased growth in all treatments and controls. The bacterial E isolate on the phenanthrene substrate at 24-hour incubation has increased growth, which occurs until the incubation time is 48 hours, both in the control and phenanthrene substrate. E Isolate is tolerant of increasing substrate concentrations up to 1000 ppm, both naphthalene and phenanthrene and has the best growth ability. It can be concluded that isolate E has the best growth ability and is tolerant of increasing substrate concentrations up to 1000 ppm. E isolates were designated as the best isolates of naphthalene and phenanthrene degradation. Keywords: Dumai, Growth Response, Naphthalene, Phenanthrene
Highlights
Pollution is one of the important problems both pollution of organic matter and inorganic materials
This study aims to find the most appropriate indigenous bacteria as Naphthalene and Phenanthrene degrading agents carried out in the growth response test
The growth response test carried out by growing 20 mL of bacteria in AMS media, which added with polyaromatic hydrocarbons (PAHs) substrate, naphthalene, or phenanthrene, with various concentrations of 200 ppm, 500 ppm, 800 ppm, and 1,000 ppm
Summary
Pollution is one of the important problems both pollution of organic matter and inorganic materials. PAH content in oil sludge reached 13.24% [3]. These compounds are one of the concerned pollutant sources because they are carcinogenic, mutagenic, and immuno-toxic. The data obtained in the form of bacterial growth response through measurement of OD every 24 hours using a spectrophotometer and TPC at 72 hours. The growth response test carried out by growing 20 mL of bacteria in AMS media, which added with PAH substrate, naphthalene, or phenanthrene, with various concentrations of 200 ppm, 500 ppm, 800 ppm, and 1,000 ppm. The data obtained were in the form of bacterial growth response through measurement of OD every 24 hours using a spectrophotometer and carried out TPC at 72 hours using Nutrient Agar media. We conducted a one-way ANOVA and Duncan statistics test to find out the difference between the treatment of the substrate treatment concentration and the incubation time
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