Abstract
The study deals with isolation of bacteria from fresh coal mine spoil and their ability to produce antimicrobial compound. In detection of antagonistic effect, one of the four bacterial isolates, strain-I exhibited maximum inhibitory effect against Escherichia coli and was selected for further study. Our results indicated, antimicrobial activity against both Gram negative (E. coli) as well as Gram positive (Staphylococcus aureus) pathogens and exhibited greater zone of inhibition against E. coli at 40 to 50°C where as against S. aureus at 40°C at the 24th hour of incubation. However, with increase in time of incubation, the zone of inhibition showed a declining trend. The antimicrobial activity of strain-I was the highest between pH 5 to 7. Culture of the isolate with glucose as a substrate resulted relatively larger zone of inhibition against E. coli at 48th hour of incubation. Key words: Strain-I, coal mine spoil, antimicrobial compound, antagonistic effect, zone of inhibition.
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