Abstract
L-arginine-nitric oxide pathway has emerged as novel regulators of several vital roles in the reproductive function which comprise pregnancy events, such as placental development. This study was done to pharmacologically enhance the performance of female reproductive system by using L-arginine powder as forerunner of nitric oxide. The study protocol consists of total number of 96 pregnant mice divided equally into two main groups (48 animals per group) and handled as follows: 1st Control group given normal saline orally daily and 2nd L-arginine dosed group 200 mg/kg BW 20% orally daily, both groups were randomly divided into four subgroup according to dosed period of pregnancy term, the dosed periods were 1-15 days, 7-15 days, 7-21 days and 15-21 days. Several parameters were evaluated and displayed the following results: L-arginine concentration in uterine tissue was elevated in association with increased body, uterine, placenta and fetus weights. That presumably was controlled by an increase food and water intakes. Hormonal levels (estrogen and progesterone) mainly at 7-21 days and 15-21 days of gestation dosed periods. Those results showed histological and stereological profile which illustrated the activity and enlargement of placental layers acquaintance with increasing blood vessels (angiogenesis and vasodilation) and vascular density (%) especially in 7-21 and 15-21 of dosed gestation periods led to an increase placental volume and geometric parameters (cm), weight (gm) and proportional thickness (cm), vascular density, and blood vessels. Fetal traits parameters, displayed significant statistical values of fetuses and weights in all gestation periods expressed at 15-21 days as the best results. Also, increases other parameters: blood volume, steriometry values, histological assessments and alkaline phosphatase and lactogens values. The endpoints of this study presented the L-arginine donated NO which was capable of increasing remodeling blood supply and improvement of some reproductive phenotypic properties of animal models and significant number of fetuses viability.
Highlights
L-arginine was an essential amino acid found in proteins of the animal body and different sources of foods
L-arginine and derived nitric oxide plays important roles in numerous biochemical reactions in the body including ammonia detoxification by formation of urea, hormonal stimulation such as pituitary stimulus lead to release of growth hormone and pancreatic release of glucagon and insulin [2] and immune modulation in which it improved the immune status [3] in those suffering from sepsis, burn and trauma [4]
L-arginine donated nitric oxide was indisputable that such a polyvalent molecule could play a decisive role in the male and female reproductive system
Summary
L-arginine was an essential amino acid found in proteins of the animal body and different sources of foods. L-arginine and derived nitric oxide plays important roles in numerous biochemical reactions in the body including ammonia detoxification by formation of urea, hormonal stimulation such as pituitary stimulus lead to release of growth hormone and pancreatic release of glucagon and insulin [2] and immune modulation in which it improved the immune status [3] in those suffering from sepsis, burn and trauma [4]. L-arginine donated nitric oxide was indisputable that such a polyvalent molecule could play a decisive role in the male and female reproductive system. The L-arginine play an important role in male fertility which were provoke penile erectile [68], enhancement sperm motility [7,9,10] and play positive position in sexual hormone regulation and ovarian function such as ovulation [11,12]. The role of NO in the uterus was suppression of myometrial contractility during pregnancy [13,14,15,16,17] in other explored extension held and fixed the facts participation of sexual and neuronal behaviors in reproductive fitness in L-arginine dosed mice [18]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call