Abstract
Objective:The objective of this study is to determine the effect of eel meat (Monopterus albus) extract ointment on an incision.Materials and Methods:The experimental animals used in this study were 20 male rats (Rattus norvegicus), Wistar, weighing 150–200 gm and aged 8–12 weeks. This study uses complete random design and is divided into four groups. In the negative control group, group treatment was carried out on the healthy rat. In the positive control group, the incision was performed without any therapy. In the T1 and T2 groups, group treatment was performed with a dose of 2% and 5% eel (M. albus) extracts. The TNF-α expression was analyzed by the immunohistochemistry (IHC) technique and epidermal thickness by Masson’s Trichrome (MT) staining. Data analysis of TNF-α expression and epidermal thickness was done using one-way analysis of variance and the Tukey test with a confidence level of 95% (α = 0.05).Results:The results showed that the eel (M. albus) extract therapy, with a concentration of 2% and 5%, significantly (p < 0.05) reduced the TNF-α expression and increased the epidermal thickness.Conclusion:It can be concluded that the administration of eel (M. albus) extract therapy could help to reduce TNF-α expression and increase epidermal thickness in rat incision wounds.
Highlights
A wound is a condition where there is damage or loss of some human body tissues and a condition where there is a loss of epithelial integrity from the skin
The results showed that the eel (M. albus) extract therapy, with a concentration of 2% and 5%, significantly (p < 0.05) reduced the TNF-α expression and increased the epidermal thickness
A wound is a breakdown or loss of cellular and anatomic continuity, with impairment of the tissue’s protective or physiological functions; trauma is a physical injury or a wound caused by external force or violence [2]; and an incision wound is cut wound caused by sharp objects that reach a certain depth, so incision wound is open wound
Summary
A wound is a condition where there is damage or loss of some human body tissues and a condition where there is a loss of epithelial integrity from the skin. Aspartic acid and glutamic acid are important for skin formation, collagen formation, the formation of pro-inflammatory cytokines, maintaining lymphocyte function, and the formation of fibroblasts and macrophages, and might suppress the inflammatory process during the wound-healing phase [6]. Wound healing can be determined by the decrease of TNF-α level and the presence of a formed epidermis layer in the injured skin [7]. TNF-α cytokine and epidermal thickness are chosen as inflammatory parameters in the incision wound-healing process from animal models. The administration of the eel extract ointment containing amino acids and fatty acids is expected to increase the thickness of the epidermis and reduce TNF-α expression. The wound will immediately undergo the proliferation stage, and, through this study, we can determine the eel extract potential on the wound-healing process based on the epidermal thickness and TNF-α expression
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