The effect of ionic silver water (Ag+) toward TNF-α (Tumor Necrosis Factor-α) expression and epidermal thickness in open wound healing rat (Rattus norvegicus) model

Abstract

Incision wounds are one type of open wounds that occur caused by a sharp instruments such as a result of medical procedure in surgery process. At the time of injury, body will naturally heal that wounds through three phases, inflammation, proliferation, and maturation (remodeling). Ag (silver) solution can accelerate wound healing because it has antibacterial properties and will increase fibroblast cells production. This study aims to determine the effect of Ag (silver) solution based on expression TNF-α (Tumor Necrosis Factor-a) and epidermal thickness in incision wound of the white rat (Rattus norvegicus) Wistar strain. The experimental animals were male white rat Wistar strains weighing 150-250 grams, aged 8-12 weeks divided into 4 groups. Negative control group without treatment. P1 group that is incised and treated by 10% Povidone Iodine with doses 0,5 ml. P2 group that incised and treated by Ag (silver) solution with doses 0,5 ml. P3 group that incised and treated by combination between 0,5 ml ag (silver) solution and 0,5 ml Povidone Iodine. All treatments was given for seven days. TNF-α expression was measured using the Flowcytometry method and epidermal thickness was observed histopathologically by Haematoxyllen Eosin (HE) staining. Data analysis TNF-α expression was performed by Kruskal Wallis test while epidermal thickness were analyzed by one way ANOVA test and tukey follow-up test with 95% confidence level (α = 0.05). The results of this study showed that combination therapy of Povidone Iodine 10% and Ag solution tends to decrease TNF-α expression and showed increasing cell regeneration of the epidermis in terms of epidermal thickness. The conclusion of this study is that combination therapy can accelerate the wound healing process.