The phosphorus source determines the arbuscular mycorrhizal potential and the native mycorrhizal colonization of tall fescue and wheatgrass

Abstract

The study assesses the effect of two phosphate (P) sources (soluble superphosphate (SP) and rock phosphate (RP)) on the arbuscular mycorrhizal potential (AMP), the root arbuscular mycorrhizal colonization (AMC) and the growth of tall fescue and wheatgrass of a grassland soil from Argentina. Mycorrhizal potential was assessed with soil samples collected from 2 years for tall fescue and wheatgrass swards before and after field plots were fertilized with 0 and 60 kg P ha −1 as SP or RP. Mycorrhizal potential both at unfertilized and at RP fertilized plots was high (12–14 AM propagules g −1), however fertilization with SP caused a decrease in AMP (0.70–0.95 AM propagules g −1). A range of soil P between 4 and 46 mg P kg −1 and a range of root AMC between 6% and 50% were obtained after fertilization with four rates of SP and RP (0, 15, 30, and 60 kg P ha −1) in plots where tall fescue and wheatgrass were grown during 2 years. Soil P and root mass were higher in the top 10-cm depth than in the 20-cm of the soil profile, but AMC did not change with depth. Shoot dry matter (SDM) production of both grasses did not differ after fertilization with SP or RP, particularly at second year. The AMP positively correlated with the indigenous AMC, and they were not different between tall fescue or wheatgrass. Lineal-plateau relationships between soil P, relative SDM and AMC were established. Highest relative SDM was attained at 6.5 mg P kg −1 in plots fertilized with RP, and at 15.2 mg P kg −1 with SP. Variability in colonization was well accounted by the soil P (at 0–10 cm depth) fertilized with SP ( r 2 = 0.48, P 0.01), but any relationship was found with RP. The AMC decreased with increasing available soil P from plots with SP until 18.3 mg kg −1 (a decrease of 2.2% per mg P kg), after that AMC was stabilized at about 6.9%. Our study clearly showed that fertilization with SP or RP produced similar available soil P content and grasses SDM production. Mycorrhiza root colonization and propagules decreased after fertilization with SP, but fertilization with RP did not decrease mycorrhizal propagules nor colonization. It can be concluded that RP fertilization instead SP could allow obtaining acceptable tall fescue and wheatgrass yield enhancing mycorrhizal potential of soils and indigenous colonization of plants and thus maximizing the use of fertilizer.