Abstract
The peroxisome (microbody) membrane differs markedly from other cytoplasmic membranes, especially the lysosomal, in its susceptibility to various disruptive treatments and detergents. Although this phenomenon has been extensively studied in biochemical literature, there have been no ultrastructural or cytochemical investigations of it. We now report on the effects of detergents, organic lipid solvents, and several adjuvants used in cell fractionation upon the ultrastructure of the peroxisomal membrane and its permeability to catalase, as demonstrated cytochemically by the DAB reaction and biochemically by the assay of catalase activity.
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