The pattern of sedimentary bacterial communities varies with latitude within a large eutrophic lake

Abstract

We investigated the diversity, composition, and assembly processes of sedimentary bacterial communities across Lake Taihu, China, a large, shallow, and eutrophic water body. Amplicon-based 16S rRNA gene high-throughput sequencing identified the composition and phylogenetic structure of the bacterial communities within the 28 collected samples. Diversity analysis revealed that sedimentary bacterial communities demonstrated significant trends with nutrient loading and habitat along latitude. We used network analysis to disentangle the role of keystone taxa in bacterial communities. Most identified keystone species were from the genus Nitrospira (affiliated with Nitrospirae), subphylum Deltaproteobacteria (affiliated with Proteobacteria), subphylum Gammaproteobacteria (affiliated with Proteobacteria), family Rhodocyclaceae (affiliated with Betaproteobacteria), phylum Bacteroidetes, genus Bacillus (affiliated with Firmicutes), and family Anaerolineaceae (affiliated with Chloroflexi) in order of abundance. These keystone taxa play fundamental roles in carbon and nitrogen cycling within Lake Taihu. Phylogenetic structure analysis indicated that the bacterial communities were more phylogenetically clustered than expected by chance and that deterministic processes dominated the assembly of bacterial communities across Lake Taihu. Niche selection was the crucial factor driving the assembly of bacterial communities. This study enhances the understanding of the distribution of sedimentary bacterial communities and their assembly mechanisms across Lake Taihu.