Abstract
Antimicrobial resistance is a global public health threat. One of the possible ways to solve this problem is phage therapy, but the instability of bacteriophages hinders the development of this approach. A bacteriophage delivery system that stabilizes the phage is one of the possible solutions to this problem. This study is dedicated to exploring methods to create encapsulated forms of bacteriophages for delivery. We studied the effect of proteolytic enzymes on the destruction of the polyelectrolyte microcapsule shell and revealed that protease from Streptomyces griseus was able to destroy the membrane of the microcapsule (dextran sulfate/polyarginine)3 ((DS/PArg)3). In addition, the protease decreased the activity of the bacteriophage in the second hour of incubation, and the phage lost activity after 16 h. It was found that a medium with pH 9.02 did not affect the survival of the bacteriophage or E. coli. The bacteriophages were encapsulated into polyelectrolyte microcapsules (DS/PArg)3. It was established that it is impossible to use microcapsules as a means of delivering bacteriophages since the bacteriophages are inactivated. When bacteriophages were included inside a CaCO3 core, it was demonstrated that the phage retained activity before and after the dissolution of the CaCO3 particle. From the results of this study, we recommend using CaCO3 microparticles as a container for bacteriophage delivery through the acidic stomach barrier.
Highlights
IntroductionAntimicrobial resistance occurs when bacteria of a certain type acquire the ability to protect themselves from antibiotics, which normally effectively disrupt their vital activity
Antimicrobial resistance occurs when bacteria of a certain type acquire the ability to protect themselves from antibiotics, which normally effectively disrupt their vital activity.It significantly complicates the treatment of infectious diseases that they cause
We examined the effect of the medium with pH = 9.02 on phage activity, which makes it possible to simulate the conditions during the formation of the CaCO3 core at the first stage of microcapsule formation
Summary
Antimicrobial resistance occurs when bacteria of a certain type acquire the ability to protect themselves from antibiotics, which normally effectively disrupt their vital activity. It significantly complicates the treatment of infectious diseases that they cause. Bacteriophages are viruses that can selectively infect the bacterial cells of one strain or antigenically homologous strains of the same species or genus. This infection is followed by lysis (after intracellular replication, except for temperate or chronic phages) of the bacterial host cell [3] and does not threaten eukaryotic cells. One of the ways to create this system is to encapsulate bacteriophages
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