The Pathological Features of Cyclophosphamide Induced Multi-Organs Toxicity in Male Wister Rats

Abstract

Cyclophosphamide has the ability to induce multi-organ toxicity. The current study was conducted to investigate the toxicity effects of one dose and two doses of intraperitoneal administration of cyclophosphamide at dose of 50 mg/kg of body weight in male Wister rats. Fifteen male rats were assigned into three group five rats each group namely, control, 1-dose, and 2-doses. 1-dose group rats received one dose of cyclophosphamide at dose of 50 mg/kg of body weight, and 2-doses group rats received two doses of cyclophosphamide at dose of 50 mg/kg of body weight. Liver, lungs, kidneys, spleen and testes weight were recorded and collected for histopathological examination. The results showed a decrement in testes and spleen weight of 2-doses treated rats. The histopathology results showed pathological changes in all examined organs of cyclophosphamide treated rat. The fibrosis lesion was observed in 2-doses group’s affected organs. Testicular injury in the 2-doses group involved 10% of the affected testis. In conclusion, cyclophosphamide treatment at dose of 50 mg/kg of body weight to male Wister rats induced multi-organs toxicity. The rats that received two doses of cyclophosphamide as dose of 50 mg/kg of body weight induced fibrosis in affected organs and elevated the testicular injury.