Abstract
The banana passion fruit (Passiflora tripartita Breiter, Passifloraceae) known as “tumbo” is very appreciated in tropical and subtropical countries of South America. Methanolic extracts from peel and the fruit juice of P. tripartita growing in Chile were analyzed for antioxidant capacity as well as for flavonoid and phenolic content. A chromatographic method was developed for the rapid identification of the main phenolics in the samples by HPLC-DAD and HPLC-MS. The fast fingerprint analysis allowed the detection of eighteen flavonoid C-glycosides and four flavonoid O-glycoside derivatives which were characterized by UV spectra and ESI-MS-MS analysis. Several of the C-glycosides detected are structurally related to the orientin derivative 4′-methoxy-luteolin-8-C-(6″acetyl)-β-D-glucopyranoside (31), fully elucidated by spectroscopic methods. The antioxidant derivative 31 along with schaftoside, vicenin II, orientin and vitexin were isolated from the fruit extract by high-speed countercurrent chromatography (HSCCC). A suitable method for the preparative isolation of flavonol C-glycosides from “tumbo” extracts by HSCCC is reported. The pulp of the fruits showed good antioxidant capacity (12.89 ± 0.02 μg/mL in the DPPH assay). The peel presented the highest content of flavonoids (56.03 ± 4.34 mg quercetin/100 g dry weight) which is related to the highest antioxidant power (10.41 ± 0.01 μg/mL in the DPPH assay).
Highlights
The genus Passiflora from the Passifloraceae plant family comprises around 450 species originated from temperate and tropical South America
The MeOH extracts of pulp and juice as well as peel from P. tripartita var. mollissima were evaluated for antioxidant power by the DPPH scavenging activity and the ferric reducing antioxidant power assay (FRAP)
The compounds responsible for the antioxidant activity of the extracts were isolated by centrifugal countercurrent chromatography (HSCCC) and were identified by spectroscopic and spectrometric means
Summary
The genus Passiflora from the Passifloraceae plant family comprises around 450 species originated from temperate and tropical South America. The best known edible Passiflora fruits are Passiflora edulis f. Flavicarpa, the purple and yellow passion fruits. The passion flower genus or passion vine is known to produce cyanogenic glycosides [1]. Passifloraceae are used worldwide in traditional medicine or in phytotherapy as anti-inflammatory [2]. Anxiolitic and sedative substances [3]. Some worldwide consumed edible fruits such as lemon [5] and lime (Citrus aurantifolia) did not contain detectable amounts of C-glycosides [6] but they occur in medicinal plants used for similar indications than Passiflora, including hawthorn
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
