Abstract
Spodoptera littoralis Boisd. Lepidoptera:Noctuidae nukleopolihedrovirus SpliNPV Baculoviridae’nin bir üyesi olup grup II NPV’leri arasında yer almaktadır. Lef-8 iyi korunmuş bir gen olup pek çok lepidopter NPV’sinde tanımlanmıştır. Kodlanan proteinlerin nükleotid ve aminoasit dizinleri ve gen içerikleri filogenetik olarak aydınlatıcı olabilmektedir. Bu çalışmada, Türk SpliNPV-B SpliNPV-TR1 genomundaki lef-8 geni PCR’la kısmi olarak çoğaltılmış ve aminoasit dizinlerine göre bazı bakulovirüslerle analiz edilmiştir. SpliNPV-TR1 kısmi lef-8 geninin aminoasit dizinleri SpltNPV ile % 84 benzelik gösterirken AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV ve BufuNPV ile sırasıyla sadece % 57, % 55 , % 51, % 50, % 50, % 50 ve % 49 aminoasit benzerliği göstermiştir. SpliNPV-TR1 ve granulovirüsler arasındaki sekanslar da düşük dizin benzerlikleri üretmiştir en fazla % 46 . Grup II NPV’leri esas olarak noctuid konukçulardan izole edilse de, bu noctuid NPV’leri arasında saptanan düşük dereceli benzerlikler SpliNPV-TR1 ve SpltNPV arasındaki yüksek benzerlik oranı hariç , bu izolatların monofiletik bir orijine sahip olamayacağını göstermektedir
Highlights
The family Baculoviridae contains invertebrate specific pathogens and is characterized by rod-shaped, enveloped virions containing a dsDNA, 90-160 kb in size (Pang et al 2001)
The amplified partial lef-8 was analyzed according to amino acid sequences. 238 aminoacids that correspond to 714 nucleotides were aligned for the partial lef-8 gene of Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) nucleopolyhedrovirus (SpliNPV)-TR1
Herniou et al (2001) recently inferred the phylogenetic relationships of nine baculoviruses using a combined data set of 63 conserved genes and the topology of this tree was identical to individual gene phylogenies from ac22, ac81, ac119, ac142, ac145, lef-8 anf lef-9
Summary
The family Baculoviridae contains invertebrate specific pathogens and is characterized by rod-shaped, enveloped virions containing a dsDNA, 90-160 kb in size (Pang et al 2001). The family is composed of two genera, nucleopolyhedrovirus (NPV) and granulovirus (GV), based on their distinct occlusion bodies (OBs) (Murphy et al, 1995). The virions are occluded in a polyhedral shaped occlusion body called polyhedra in the NPV genus and are protected against enviromental conditions for years (Toprak et al 2005a). Gene expression in the baculoviruses has been extensively studied in the Autographa californica Speyer (Lepidoptera:Noctuidae) MNPV, and four phases of expression were classified: immediate-early, delayedearly, late and very late (Friesen and Miller 1986).
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