Abstract
Background KLF4 and KLF5 are members of the KLF transcription factor family, which play an important role in many gastrointestinal tumors. To gain a deeper insight into its function and role, bioinformatics was used to analyze the function and role of KLF4 and KLF5 in gastrointestinal tumors. Methods Data were collected from several online databases. Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN database analysis, Kaplan-Meier Plotter analysis, LOGpc system, the Pathology Atlas, and the STRING website were used to analyze the data. We download relevant data from TCGA and then perform GO enrichment and KEGG enrichment analysis. The effects of KLF5 on gastric cancer cell proliferation were measured by CCK-8 assay. The effect of KLF5 on the expression of CyclinD1 and MMP9 was detected by Western blot. Results KLF4 and KLF5 were differentially expressed in normal and tumor tissues of the gastrointestinal tract, and their differential expression is related to several genes or pathways. KEGG analysis showed that KLF5 was coexpressed with endocytosis-related genes. KLF5 promotes the proliferation of gastric cancer cells and the expression of metastasis-related molecules. Conclusion KLF4 and KLF5 are of great significance for developing gastrointestinal tumors and can be used as therapeutic targets.
Highlights
Malignant tumors are common diseases that significantly threaten human health, and transcription factors play an important role in tumor occurrence and development
To assess the correlation between Krüppel-like factor 4 (KLF4) and Krüppel-like factor 5 (KLF5) and clinical disease, UALCAN, Gene Expression Profiling Interactive Analysis (GEPIA), and Kaplan-Meier Plotter were used for prognostic survival analysis
There was no significant difference (P > 0:05), we observed that the high expression of KLF5 was negatively correlated with the survival rate of patients according to the trend
Summary
Malignant tumors are common diseases that significantly threaten human health, and transcription factors play an important role in tumor occurrence and development. The KLF/SP family is expressed in various tissues and has different tissue-specific activities and functions [2]. These genes regulate physiological processes such as embryogenesis, growth, development, differentiation, and proliferation and regulate the pathogenesis of many diseases, including inflammatory diseases and cancers [3,4,5,6]. The KLF/SP transcription factor family members can regulate their homeostasis and pathophysiology. KLF4 and KLF5 are members of the KLF transcription factor family, which play an important role in many gastrointestinal tumors. KLF4 and KLF5 are of great significance for developing gastrointestinal tumors and can be used as therapeutic targets
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