Abstract
Unsaturated fatty acid methyl esters and triglycerides were separated as their silver ion π-complexes by reversed-phase partition chromatography in the system 70–100% aqueous methanol, saturated with silver nitrate and dodecane/dodecane. The fatty acid composition of single fractions was determined by gas-liquid chromatography. The unsaturated methyl esters were completely separated from saturated ones of the same polarity, and from unsaturated esters with different carbon atom or double bond number and different position of the double bonds in the aliphatic chain. Triglyceride fractions of the same polarity were fractionated into individual compounds according to double bond or saturated fatty acyls number. The method may be used on an analytical or preparative scale.
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