The p33 ING1b tumor suppressor cooperates with p53 to induce apoptosis in response to etoposide in human osteosarcoma cells

Abstract

p33 ING1b induces cell cycle arrest and stimulates DNA repair, apoptosis and chemosensitivity. The magnitude of some p33 ING1b effects may be due to activation of the tumor suppressor p53. To investigate if the p33 ING1b protein affected chemosensitivity of osteosarcoma cells, we overexpressed p33 ING1b in p53+/+ U2OS cells or in p53-mutant MG63 cells, and then assessed for growth arrest and apoptosis after treatment with etoposide. p33 ING1b increased etoposide-induced growth inhibition and apoptosis to a much greater degree in p53+/+ U2OS cells than in p53-mutant MG63 cells. Moreover, ectopic expression of p33 ING1b markedly upregulated p53, p21 WAF1 and bax protein levels and activated caspase-3 protein kinase in etoposide-treated U2OS cells. Together, our data indicate that p33 ING1b prominently enhances etoposide-induced apoptosis through p53-dependent pathways in human osteosarcoma cells. p33 ING1b may be an important marker and/or therapeutic target in the prevention and treatment of metastatic osteosarcoma.