Abstract

The unconventional yeast Yarrowia lipolytica is used to produce erythritol from glycerol. In this study, the role of the erythrose reductase (ER) homolog YALI0B07117g in erythritol synthesis was analyzed. The deletion of the gene resulted in an increased production of mannitol (308%) and arabitol (204%) before the utilization of these polyols began. The strain overexpressing the YALI0B07117g gene was used to increase the erythritol yield from glycerol as a sole carbon source in batch cultures, resulting in a yield of 0.4 g/g. The specific consumption rate (qs) increased from 5.83 g/g/L for the WT strain to 8.49 g/g/L for the modified strain and the productivity of erythritol increased from 0.28 g/(L h) for the A101 strain to 0.41 g/(L h) for the modified strain. The application of the research may prove positive for shortening the cultivation time due to the increased rate of consumption of the substrate combined with the increased parameters of erythritol synthesis.

Highlights

  • The increase in sugar and fat consumption has led to a drastic rise in overweight and obese individuals

  • As the role of this sequence remains unknown, it probably does not possess any significance in erythritol synthesis

  • The production of erythritol synthesis byproducts decreased compared with both the WT strain and the knock-out mutant (Table 1). These results suggested the potential for an increase of erythritol synthesis with glycerol as the main carbon source

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Summary

Introduction

The increase in sugar and fat consumption has led to a drastic rise in overweight and obese individuals. Obesity increases the risk of diabetes, cardiovascular diseases and certain types of cancer [1,2]. To avoid the health issues resulting from excessive sugar consumption, sugar substitutes have been implemented. Sugar alcohols, are food additives that occur naturally in nature, especially fruit and vegetables [1,3]. The application of polyols as sweeteners became possible due to their industrial production. The constantly rising demand for polyols necessitates further improvement in the yield on an industrial scale

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