Abstract
Objective: To evaluate fertilization rate, embryo quality and pregnancy rate in patients undergoing Intracytoplasmic Sperm Injection (ICSI) using fresh and cryopreserved ejaculated sperm. Methods: We carried out an analytical cross-sectional study with retrospective data collection from December 1st, 2017 to May 1st, 2018. We compared two groups of patients undergoing ICSI using ejaculated sperm: group I comprised of those in whom fresh semen was used and group II was comprised of those in whom cryopreserved semen was used. We collected the fertilization rate, embryo quality, and pregnancy rate. The Odds Ratio was calculated to determine the association between the variables. A p-value of <0.05 was considered significant. A logistic regression analysis was done. Results: when OATS sperm was used, we had higher fertilization rate 67, 5% vs. 46, 9%, p=0,042 and better embryo quality on day 3, 95% vs. 5%, p=0.008 with freshly ejaculated sperm against the cryopreserved semen. After logistic regression, the fertilization rate, embryo quality and pregnancy rate in the fresh and cryopreserved ejaculated semen were similar. Conclusion: The semen with OATS may be more susceptible to damages caused by cryopreservation, resulting in lower fertilization rate and embryo quality but similar pregnancy rates.
Highlights
Our objective was to evaluate the effect of sperm cryopreservation on fertilization rate, embryo quality and pregnancy rate in patients undergoing Intracytoplasmic Sperm Injection (ICSI) using either fresh or cryopreserved ejaculated sperm
Recent studies showed no difference in terms of fertilization rate and embryo quality after ICSI with either fresh or cryopreserved ejaculated sperm used [1]
Semen with OATS may be more susceptible to sperm cryopreservation damage, resulting in lower fertilization rate and embryo quality
Summary
Since the introduction in 1953 and 1954 of a simple "Dry Ice" sperm cryopreservation method which maintained fertilizing capacity and resulted in normal progeny, the need of developing sperm banking has become paramount [1,2,3,4,5,6,7,8,9,10].The role of this method of preserving fertility is to overcome the difficulty of having sperm available the day of oocyte retrieval in patients undergoing Assisted Reproductive Technique (ART) to treat infertility.More than in only medical indications like azoospermia, the ability to cryopreserve sperm before undergoing ICSI allows more flexibility to the male partner. Since the introduction in 1953 and 1954 of a simple "Dry Ice" sperm cryopreservation method which maintained fertilizing capacity and resulted in normal progeny, the need of developing sperm banking has become paramount [1,2,3,4,5,6,7,8,9,10]. The role of this method of preserving fertility is to overcome the difficulty of having sperm available the day of oocyte retrieval in patients undergoing Assisted Reproductive Technique (ART) to treat infertility. It is responsible for some membrane distortion and possible DNA abnormalities [7,8]
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