Abstract
AbstractOsmium tetroxide bipyridine ([OsO4(bpy)]) is a versatile label for DNA electrochemistry. Here we report our efforts to create an osmium tetroxide‐labeled immobilized DNA probe for use in biosensing experiments. Our label is applied in‐house, as opposed to many other covalent redox labels used with DNA. We developed an on‐electrode labeling method that was able to avoid attack of our disulfide‐based linker by the [OsO4(bpy)]. Our results include two different hairpin‐based signal‐off hybridization detection assays, each with robust, reproducible signal decrease on binding to complementary target. We also found that 6‐mercapto‐1‐hexanol was able to interact with [OsO4(bpy)] in a stable manner when assembled at the surface, but was able to remove excess [OsO4(bpy)], which was found to adsorb strongly to the gold surface, and avoid MCH transformation, when applied after the labeling process. Furthermore, we found an increased adsorption affinity of [OsO4(bpy)]‐labeled DNA onto gold, characterized the redox behavior of adsorbed [OsO4(bpy)], and determined voltammetric signals on the HMDE of our intact disulfide linker while verifying that this linker is attacked by [OsO4(bpy)] when in solution.
Published Version
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