Abstract
A total of 262 water-related samples were analysed for the presence of Cryptosporidium spp. oocysts, of which 39.3% were positive, over a twelve month period. Similar data were obtained for their occurrence in samples of both raw and treated water from Scotland, with 40.5% of raw water and 40.1% of treated water being positive. Fewer oocysts were detected in the summer than in the autumn, winter or spring. In a 12 month survey of water used for abstraction, oocysts were detected at various times throughout the year in both raw and treated water. A series of conventional and fluorogenic dyes was used, in conjunction with a fluorescent labelled monoclonal antibody, in an attempt to assess their potential fox imprpving the identification of oocysts in water-related samples. In addition, preliminary data regarding the possible use of fluorogenic vital dyes as indicators of in vitro excystation and viability are presented.
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