The Occurrence of Choline Kinase II in the Cytoplasm of Soybean Root Nodules Infected with Various Strains of Bradyrhizobium japonicum

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Summary The soluble nodule-specific protein choline kinase II (CK II) accounted for 37% – 49 % of the total choline kinase activity in those symbioses where stable peribacteroid membranes (PBM) are made. The induction of choline kinase II was independent of whether the bacteria were nif + or fix − . A fix − strain which does not build stable PBM (strain 61-A-24) could not induce the production of CK II. The mutant RH 31-Marburg (fix-) which builds PBM which fuse to give larger vacuoles, induced CK II but to a lesser extent (25 % – 43 % of total activity) than the wild type. Choline kinase II induction for each bacterial strain was the same irrespective of soybean cultivar.