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Abstract
The nucleotide sequence of the region between map position 4.5 ( HpaI-site) and 8.0 ( HindIII-site) of adenovirus type 5 (Ad5) DNA has been determined. This stretch of DNA is part of the transforming HindIII-G fragment, which is 2809 nucleotides long. The sequenced segment was found to have a long open reading frame for protein biosynthesis, starting 23 nucleotides from the HpaI site and extending all the way to the HindIII-G site, which could code for a protein of at least 44 000 daltons. The possible correlation between the coding capacity of the HindIII-G fragment and the “transforming” proteins specified by it will be discussed in the light of the recent data on the splicing of early mRNAs.
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