The nucleotide sequence of adenovirus type 5 early region El: the region between map positions 8.0 ( HindIII site) and 11.8 ( SmaI site)

Abstract

The nucleotide sequence of the region between map positions 8.0 ( HindIII site) and 11.8 ( SmaI site) of adenovirus type 5 (Ad5) has been determined. Together with the sequences reported earlier (Van Ormondt et al., 1978; Maat and Van Ormondt, 1979) it encompasses the entire leftmost early region El of Ad5 DNA (4126 base pairs). The total sequence revealed a number of potential regulatory signals (promoter sites, ribosome binding sites, 3′-poly(A)-associated sequences), which confirm that region El is divided into subregions, Ela and Elb, and a region coding for semi-late viral protein IX. By taking into account the adenovirus 2 (Ad2) RNA-splicing data of Perricaudet et al. (1979; 1980) and the Ad2 RNA mapping data of Chow et al. (1979) we predict that Ela codes for polypeptides of 32, 26 and ca. 13 kd, and Subregion Elb for polypeptides of 67 kd and 20 kd; the expected molecular weight of protein IX is 14.4 kd.