Abstract
Nuclei and chromatin are rarely studied at a physiological salt concentration since they aggregate so readily [16]. As a result, they are generally studied in the presence of “stabilizing” divalent cations under hyper- or hypotonic conditions. Such conditions are unsatisfactory for several reasons. The “stabilizing” cations activate nucleases, destroying template integrity and supercoiling, and unphysiological salt concentrations may introduce artefacts. It has been suggested that structures called variously the nuclear matrix, cage or scaffold, are the site of replication and transcription [8], but they are not seen in the micrographs of “genes in action” obtained by Miller and colleagues using hypotonic conditions [15, 14].
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
