Abstract
The nitrous oxide (N 2O) reductase ( nos) gene cluster from Achromobacter cycloclastes has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ (structural N 2O reductase gene), nosD, nosF, nosY, nosL, and nosX are detected, indicating a genetic organization similar to that of Rhizobium meliloti. To aid homology studies, nosR from R. meliloti has also been sequenced. Comparison of the deduced amino acid sequences with corresponding sequences from other organisms has also allowed structural and functional inferences to be made. The heterologous expression of NosD, NosZ (N 2O reductase), and NosL is also reported. A model of the Cu A site in N 2O reductase, based on the crystal structure of this site in bovine heart cytochrome c oxidase, is presented. The model suggests that a His residue of the Cu A domain may be a ligand to the catalytic Cu Z site. In addition, the origin of the spectroscopically-observed Cys coordination to Cu Z is discussed in terms of the sequence alignment of seven N 2O reductases.
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