The Network Pharmacological Mechanism of Yizhiningshen Oral Liquid in the Treatment of Tic Disorders

Abstract

Objective To predict the mechanism of Yizhiningshen Oral Liquid in the treatment of tic disorders (TD) through network pharmacology method and molecular docking technology. Methods TCMSP database, ETCM database, Batman-TCM database, Pubchem database and Swiss Target Prediction were used to collect the active ingredients of Yizhiningshen liquid and their targets were predicted. The targets of TD were screened out through GeneCards, TTD and Drugbank databases, and Venny2.1.0 was used to obtain the targets intersection. Then, a Venn diagram was drawn. Cytoscape was applied to construct the “drug-ingredients-targets” network of Yizhiningshen liquid for the treatment of TD. We built the target protein interaction network through the STRING database and performed the GO function enrichment analysis of key targets and the KEGG pathway enrichment analysis. The interaction between the active ingredients of the drug and the disease target was verified by Autodock Vina software. Results 39 active ingredients and 61 drug-disease common targets of Yizhiningshen oral liquid in the treatment of TD were screened, and its mechanism of action was inferred to be relevant to mediating 117 signaling pathways such as neuroactive ligand-receptor interaction, GMP-PKG, calcium signaling pathway, adrenergic signaling in cardiomyocytes, DA synapse, etc. Effective ingredients (Onjixanthone I, 3,4,5-Trimethoxy Cinnamic Acid and 7-O-methylisomucronulatol) have good docking results with target (PTGS2). Conclusion The active ingredients of Yizhiningshen oral liquid may treat TD through regulating anti-inflammatory response, central neurotransmitter and hormone levels.