The NADPH-oxidase LsRbohC1 plays a role in lettuce (Lactuca sativa) seed germination

Abstract

Reactive oxygen species (ROS) are increasingly recognized as non-enzymatic players in the processes of radicle elongation growth and endosperm weakening during seed germination. NADPH oxidases (EC 1.6.3.1), also known as respiratory burst oxidase homologues (Rbohs), are key enzymes for the production of ROS. We previously reported that ROS are involved in the radicle elongation growth and endosperm weakening during lettuce seed germination. However, the function of the gene(s) encoding Rbohs during lettuce seed germination remains to be elucidated. In this study, one lettuce Rboh gene LsRbohC1 was cloned, and over-expression and RNAi-lines of this gene were generated. It was found that LsRbohC1 was abundantly expressed in germinating seeds, especially in the endosperm cap and hypocotyl. Over-expression/knock-down of this gene observably increased/decreased the production of superoxide radicals in the radicle and endosperm cap, and significantly promoted/delayed the germination process. The results suggest that LsRbohC1 plays a role in lettuce seed germination.