Abstract
The rodenticides brodivacoum, difenacoum, coumatetralyl and warfarin are determined in animal relicta by high-performance exclusion chromatography on porous silica. The first three compounds are not separated, but are subsequently differentiated by adsorption or reversed-phase high-performance liquid chromatography of the appropriate eluate fraction collected from the exclusion column. The method is rapid, and clean-up (on Sep-Pak silica cartridged) is simple. Mean recoveries from spiked substrates were generally above 80% at levels of 0.1–1.0 mg/kg. Routine limits of determination are about 0.05–0.1 mg/kg for warfarin and about 0.02 mg/kg for the other compounds. If analysis for warfarin is not required, the latter limit can be lowered to about 1 μg/kg by a slight modification to the clean-up step.
Published Version
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