The Motor Kinesin 4II Is Important for Growth and Chloroplast Light Avoidance in the Moss &lt;i&gt;Physcomitrella patens&lt;/i&gt;

Daniel Macveigh-Fierro,Erkan Tüzel,Luis Vidali

doi:10.4236/ajps.2017.84055

Abstract

Understanding how plant cells adapt dynamically to changes in the environment is a fundamental problem of plant biology. Under many conditions, plant cells respond to environmental changes by modifying their intracellular organization. A critical example of intracellular reorganization is chloroplast photo-relocation, which is required for optimal energy harvesting and avoiding photodamage. A key system responsible for the spatial organization of intracellular components is the microtubule cytoskeleton and its associated motor proteins, kinesins. Here we tested the hypothesis that members of the kinesin 4II subfamily are important for chloroplast photo-relocation in the moss Physcomitrella patens. Most land plants, including P. patens, use an actin cytoskeleton-dependent mechanism to transport chloroplasts in response to light. In addition to the actin-based system, P. patens can also transport chloroplasts via a microtubule-dependent mechanism, which is absent in flowering plants. Here, we used a P. patens line that contains an inducible RNAi system to silence all three kinesin 4-II genes present in this moss and evaluated their participation in the microtubule-dependent chloroplast light avoidance response. Because we found a significant effect on cell growth when kinesin 4IIs are silenced, we took advantage of the inducible system to establish a reproducible and quantitative assay to evaluate chloroplast photo-relocation in full-grown cells. Using a laser scanning confocal-based chloroplast light avoidance response assay, we found a reduction in chloroplast motility when kinesin 4IIs were silenced. Hence, in addition to identifying a role for kinesin 4II proteins in protonemal cell growth, our results strongly support the hypothesis that these kinesins play an important role in the chloroplast light avoidance response.

Highlights

Chloroplasts are organelles that can be found in most plant and algal cells
In the present work we investigate the participation of kinesin 4IIs in the chloroplast light avoidance response
We selected kinesin 4IIs because we had previously determined that they are present in P. patens but absent from A. thaliana [32]; this absence is similar to the microtubule-based chloroplast motility present in the moss P. patens, but absent in A. thaliana

Summary

Introduction

Chloroplasts are organelles that can be found in most plant and algal cells. They are essential for photosynthesis and the survival of plant and algae species. In these cells, chloroplasts have the ability to move to different locations within the cell depending on the light intensity they are exposed to [1] [2] [3]. These avoidance and accumulation responses are driven by blue light, but in the moss Physcomitrella patens, chloroplasts have been shown to respond to red light [6] [7] [8]. What is clearly established is that, downstream from the light signal, in all vascular plants the actin cytoskeleton plays an important role in chloroplast movement [12] [13], while in P. patens the microtubule cytoskeleton participates in chloroplast transport [8] [14]

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