Abstract
AbstractPrimary explants from Rana catesbeiana tadpole liver have been maintained in organ culture for six days using methods of cultivation which are described in detail. The organization and morphology of the cells in culture have been defined by light and electron microscopy. By adjustment of the experimental conditions of culture, after six days, the cells are morphologically identical to those freshly excised from tadpole liver. Soon after being put into culture, however, the chromatin of the nuclei becomes condensed, and only after about 30 hours in culture do the original euchromatic characteristics of the nucleus reappear. The effects of changes in the culture conditions on these morphological characteristics are presented.The system that has been developed is discussed with a view toward elucidating the basis of the cytological changes which occur during anuran metamorphosis.
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