Abstract
The composition of free and protein amino acids, patterns of glucose-U-C 14 labeling, and amino acid pools of various tissues of Rana catesbeiana tadpoles and frogs were compared. It was demonstrated that there were no significant variations in the amino acid composition of whole organ proteins within one animal and of corresponding tissues of tadpole and frog. However, marked differences in several free amino acids in muscle were observed, with histidine being unusually abundant. Glucose-U-C 14 was incorporated into the protein amino acids alanine, glutamic acid, and aspartic acid in all tadpole tissues; in addition to these compounds, serine, glycine, and proline were also labeled in tadpole liver. Radioactivity levels of the free amino acids indicated that the synthesis of serine and glycine from glucose occurred more readily in frog liver than in tadpole liver. The time course of glucose-U-C 14 utilization for the formation of free and protein amino acids in tadpole liver was followed. It was observed that the maximum radioactivity in the free amino acids was reached before the peak of radioactivity was seen in the protein amino acids.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
