THE MORPHOLOGICAL CHARACTERISTICS OF ADAPTED LAMTORO (Leucaena leucocephala CV TARRAMBA) pH 3.4 THAT PRODUCED BY 40GY GAMMA RAY IRRADIATION BASED ON DIFFERENCES OF CYTOKININS IN TISSUE CULTURE

Anandia A,Aryanto A T,Karti P D M H,Prihantoro I

doi:10.24843/pastura.2019.v08.i02.p01

Anandia A, Aryanto A T + Show 2 more

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https://doi.org/10.24843/pastura.2019.v08.i02.p01

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Abstract

Lamtoro, a legume tree plant with high protein content that has a wide range of environmental adaptation to dry stress, but relatively limited to acid stress. Mutation of lamtoro through 40 Gy gamma ray irradiation on callus culture produces lamtoro callus candidates adapted with pH 3.4. Aim of the study was to measure the morphological characteristics of adapted lamtoro cv Tarramba pH 3.4 that produced by 40Gy gamma ray irradiation based on differences of cytokinins in tissue culture. The study used complete randomized design with three treatments of different sources of cytokines i.e. kinetin, BAP and TDZ with 10 replications. The results showed that 0.5 ppm TDZ was the best treatment in callus diameter, and produced crumbly callus texture, but showed callus color response that tended to be light green.

Highlights

Lamtoro, a legume tree plant with high protein content that has a wide range of environmental adaptation to dry stress, but relatively limited to acid stress
The results showed that 0.5 ppm TDZ was the best treatment in callus diameter, and produced crumbly callus texture, but showed callus color response that tended to be light green
Saran Saran dari penelitian ini adalah (1) perlu dilakukan optimasi level TDZ untuk dihasilkan dominasi kalus bertekstur remah dan bersifat embriogenik, (2) Lebih lanjut berorientasi pada regenerasi kalus untuk dihasilkan eksplen tanaman yang sempurna

Summary

Introduction

A legume tree plant with high protein content that has a wide range of environmental adaptation to dry stress, but relatively limited to acid stress. Glutamin merupakan salah satu jenis asam amino yang paling banyak digunakan pada kultur jaringan tanaman untuk berbagai tujuan, baik untuk induksi pembentukan maupun pertumbuhan kalus embriogenik (Das dan Mandal, 2010), induksi pembentukan dan proliferasi tunas (Patil et al, 2009), serta peningkatan kualitas (Wang et al, 2007). Penelitian ini bertujuan untuk mengukur karakteristik morfologi kalus lamtoro cv Tarramba teradaptasi pH 3.4 hasil iradiasi sinar gamma 40Gy berdasaarkan perbedaan sumber sitokinin (kinetin, BAP, TDZ) pada kultur jaringan.

Results

Conclusion