The morphological changes in unmyelinated peripheral nerve fibers exposed to low sodium and high potassium concentrations

Abstract

Frog sciatic nerves were incubated in vitro in isotonic solutions in which the relative concentrations of sodium and potassium were varied. The cross-sectional areas of the unmyelinated axons, the Schwann cells ensheathing the axons, and the periaxonal spaces were measured, and the densities of the axoplasmic organelles were determined. It was found that the nerve fibers were relatively resistant to solutions with low sodium and high potassium concentrations. At very low sodium and high potassium concentrations, there was swelling of the Schwann cells with compression of the axons. A slight degeneration of the axoplasmic organelles was also noted. In an extremely low sodium and high potassium solution, the mesaxons of a few nerve fibers opened up; this was associated with swelling of the axons and slippage of the opposing surfaces of the axolemma and Schwann cell membrane. The width of the periaxonal space remained unchanged even with extreme shrinkage or swelling of the axons. These findings indicate that the Schwann cell and axon react differently to low sodium and high potassium concentrations and that the Schwann cell appears to act as a buffer zone, protecting the axon by maintaining optimal ionic concentrations in the periaxonal space.