The morphokinetic effects of culture media with low lactate during early embryonic development

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To investigate the morphokinetic effects of culture media in early embryonic development. Retrospective randomized study. Data used in this study were collected from our routine IVF-PGT patients who used either autologous and/or donor oocytes between February and December 2018. All cases whose embryos were incubated in conventional incubators were excluded. Emrbyoscope slides were prepared using two different media where well 1-6 contained one media vs. well 7-12 had the second media and equilibrated overnight. On the day of retrieval, patients with at least 10 MII oocytes were randomly selected for this study. After ICSI, MII oocytes (n=35 patients, n=404 MII oocytes) were divided into two groups, and cultured in the same embryoscope (Vitrolife) slides including two different media; Group #1 (Global total by Life global) vs Group #2 (Low lactate: 1mM, CSCM-NX by Irvine). The embryos were hatched on Day 3 and trophectoderm biopsies (n=212) were performed accordingly. The biopsied materials were sent to CooperGenomics for Next Generation Sequencing [NGS] testing. There was no bias between two groups in this study because, the sibling oocytes were incubated under the same condition. Morphokinetic parameters were analysed using t-test, Fertilization, Irregular division, Blastulation and Euploidy rates were analysed using χ2-test among the groups. According to our findings, all results were shown in Table 1. The differences were considered statistically significant once p-values are < 0.05.Tabled 1GroupsGroup 1Group 2p-valueNumber of MII202202N/AtPB23.6 ± 0.93.9 ± 1.10.15tPNa7.2 ± 1.27.8 ±2.30.2tPNf24.6 ±3.325.3 ±2.60.12t226.9 ±3.729.0 ±4.10.01*t337.0 ±4.438.7 ±4.30.02*t439.7 ±4.640.9 ±5.50.07t550.0 ±5.451.1 ±6.20.31cc2 (t3-t2)9.7 ±3.49.9 ±2.30.68s2 (t4-t3)2.3 ±2.51.8 ±3.10.44tM95.0 ±8.094.6 ± 9.00.84tSB105.0 ±8.3102.2 ± 8.30.03*tB111.4 ±9.7109.9 ± 8.30.38tEB117.9 ±8.4119.2 ± 10.60.31Fertilization (%)81.80%80%0.68Euploidy (%)36%42%0.09Aneuploidy (%)45%31%0.11Mosaicism (%)17%22%0.11Irregular Division (%)17%21%0.27Day 5 Blastulation (%)58%52%0.38 Open table in a new tab In this study, we only demonstrated that the t2 and t3 divisions were earlier, and blastulation was later in Group #1 than Group #2. Although the euploidy rate was higher in media with low lactate (Group #2), this difference was not statistically significant. In conclusion, a larger sample size is needed to conclude the positive effects of culture media with low lactate.