The Molecular Identification of Sea Fungus Isolates from Mangrove Litter as Antimicrobials Potential

Abstract

Marine microorganisms, one of which is sea fungus, have the potential to produce active metabolites which have pharmacological effects. This study aims to identify pure fungus isolates from mangrove litter and tested its antimicrobial activity on bacteria Escherichia coli, Staphylococcus aureus and fungus Candida albicans. In identification result of molecular using primary Internal Transcribed Spacer, Penicillium biourgeianum species were obtained in sequences DNA CTGGGTCAC…TAT. Sea fungus isolates later are fermented using a shaker at a speed of 150 rpm for 21 days and separated between the media and mycelium. The media was extracted liquid and the mycelium was macerated using ethyl acetate. Screening test of antimicrobial activity obtains the result showing antimicrobial activity against the tested microbes. Media culture and mycelium extracts which have antimicrobial activity are then determined by the MIC and MBC values. Most of the MIC test values are 256 μg/mL, which provide strong inhibitory. MBC value gained is 512 μg/mL showing a strong bactericidal. Study result shows that sea fungus isolates produce active metabolites that mostly have antimicrobial activity against Staphylococcus aureus ATCC29737, Escherichia coli ATCC10536, and Candida albicans ATCC10231.