Abstract
The crane fly Tipula (Formotipula) melanomera gracilispina belongs to the Tipulidae. The mitogenome of T. (F.) melanomera gracilispina was sequenced, the new representative of the mitogenome of the family. The nearly complete mitogenome is 14,575 bp totally, consisting of 13 protein-coding genes, two rRNAs and 22 transfer RNAs. All genes have the similar locations and strands with that of other published species of Tipuloidea. The nucleotide composition biases toward A and T, which together made up 76.3% of the entirety. Bayesian inference analysis strongly supported the monophyly of Tipuloidea. It suggested that monophyletic Tipulidae was assigned to the sister to the monophyletic Cylindrotomidae and the Limoniidae is the sister group to the clade of Tipulidae + Cylindrotomidae.
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