Abstract
The crane fly Conosia irrorata belongs to the subfamily Limnophilinae of Limoniidae. The mitogenome (GenBank accession number: MK864103) of C. irrorata was sequenced, the new representative of the mitogenome of the subfamily. The nearly complete mitogenome is 14,634 bp totally, consisting of 13 protein-coding genes, two rRNAs, and 22 transfer RNAs. All genes have the similar locations and strands with that of other published species of Limoniidae. The nucleotide composition biases toward A and T, which together made up 77.5％ of the entirety. Bayesian inference analysis strongly supported the monophyly of Tipuloidea. It suggested that the monophyletic Tipulidae was assigned to the sister to the monophyletic Cylindrotomidae. The Limoniidae is the sister group to the clade of Tipulidae + Cylindrotomidae, and Pediciidae was assigned to the sister to the group that contain Limoniidae + (Tipulidae + Cylindrotomidae).
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