Abstract
Abstract In this study, we aim to identify the expression patterns of microRNA (miR)-424 and miR-503 in cervical cancer, with a specific focus on the malignant phenotypes in this cancer. Transfected human cervical cancer cells were assayed for their proliferation, autophagy, migration and invasion using EdU flow cytometry, MDC staining, and transwell chamber systems. Expression of epithelial-mesenchymal transition (EMT)-specific markers (E-cadherin, N-cadherin and vimentin) was determined by immunofluorescence and Western blot assays. Tumorigenesis of human cervical cancer cells was evaluated in nude mice. It was established that miR-424 and miR-503 formed a miRNA cluster that was downregulated in cervical cancer cells. Elevated expression of miR-424/miR-503 was found to inhibit cervical cancer cell proliferative, migratory and invasive potential, EMT, and tumorigenesis, but to facilitate the autophagy. CCND1 was verified as the target gene of miR-424/miR-503, based on luciferase activity assay. Upregulation of miR-424/miR-503 was shown to negate the effect of CCND1 overexpression on the malignant phenotypes in cervical cancer. These results characterized that the miR-424/miR-503 cluster prevents the malignant phenotypes and limits cervical cancer metastasis by repressing CCND1 expression.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
