Abstract
Head and neck squamous cell carcinoma (HNSCC), which occurs frequently worldwide, is characterized by high risk of metastasis. MicroRNAs (miRNAs) play crucial roles in tumorigenesis and cancer development. In this study, miR-29c-5p was identified using three high throughput microarrays. We measure miR-29c-5p expression in HNSCC tissues and cell lines. To determine the function of miR-29c-5p in HNSCC, we evaluated its effects in vitro on cell proliferation, the cell cycle, apoptosis, and cell migration. We employed a mouse tumor xenograft model to determine the effects of miR-29c-5p on tumors generated by HNSCC cell lines. The miR-29c-5p expression was lower in HNSCC tissues than in normal tissues. Upregulated miR-29c-5p expression in HNSCC cells inhibited migration and arrested cells in the G2/M phase of the cell cycle. Further, upregulated miR-29c-5p expression inhibited the proliferation of HNSCC cells in vivo and in vitro. In addition, transmembrane protein 98 (TMEM98) was identified as a direct target of miR-29c-5p by using a luciferase reporter assay. These findings provide new insights that link the regulation of miR-29c-5p expression to the malignant phenotype of HNSCC and suggest that employing miR-29c-5p may serve as a therapeutic strategy for managing patients with HNSCC.
Highlights
Head and neck squamous cell carcinoma (HNSCC) is one of the most frequently diagnosed carcinomas in Head and Neck Surgery [1, 2]
We analyzed the expression of these three microRNAs in our cohort and found the expression level of miR-29c5p was significantly diminished in HNSCC tissues (0.483 ± 0.015) compared with normal tissues in our cohort (1.000 ± 0.023; P
The results indicate that cell proliferation rates and the percentages of S-phase cells were significantly decreased in cells transfected with the miR-29c-5p mimic, while these values were increased after cotransfection with TMEM98overexpression plasmid (Figure 6C–6F)
Summary
Head and neck squamous cell carcinoma (HNSCC) is one of the most frequently diagnosed carcinomas in Head and Neck Surgery [1, 2]. 70% of patients with HNSCC in developed countries suffer from metastasis [3]. Despite advances in the development and implementation of screening technologies, HNSCC is frequently diagnosed, and its associated mortality and social economic burden are sharply rising worldwide [3, 4]. Increasing evidence demonstrates that the pathogenesis of HNSCC involves numerous factors such as genetic alterations, molecular function, ethnicity, environment, and hormone levels [3, 7, 8]. This information contributes to earlier diagnosis, improved strategies for administering individualized treatment and accurate predictions of prognosis, there is limited availability of prognostic markers, such as molecular alterations, to manage aggressive tumor progression or to implement individualized therapeutic regimens for patients with HNSCC [6]
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