THE METHOD OF CHEMICAL FERMENTATION IN ARTIFICIAL GASTROINTESTINAL JUICE OF ECHINOCOCCUS MULTILOCULARIS LARVOCYST AS A METHOD FOR OBTAINING A URIFIED VIABLE PROTOSCOLEX CULTURE

Abstract

For the first time in the world, a pure viable culture of E. multilocularis protoscolexes was isolated by digesting the parasite's larvocysts in artificial gastric juice. The article describes a unique biological, biochemical method for obtaining a culture of E. multilocularis protoscolexes purified from the connective and other tissues of the parasite larvocysts, including dead/immature protoscolexes during isolation. Methods based on the digestion of tissues in artificial gastric juice (IGF) have proven themselves well, especially in the laboratory diagnosis of trichinosis. Mixed with IGS at the rate of 1:10 (1 g of larvocyst fragments per 15 ml of solution) of the following composition: tap water – 1000 cm3 (temperature 41–42 oC); concentrated hydrochloric acid (specific gravity 1.2) – 10 cm3; food pepsin – 6.0 g; NaCl – 9.0 g. The developed method guarantees the production of viable protoscolexes of the parasite. The viability of the isolated protoscolexes was assessed by sediment microscopy with a 4×10 lens magnification for their integrity, locomotor activity, and the presence/absence of foreign matter. The method is simple to perform, does not require additional equipment and high material costs, allows you to increase the yield of pure viable E. multilocularis protoscolexes up to 95–98%.