The metabolomic approach to the complex biochemical characteristics of cole <i>Brassica oleracea</i> L.

Abstract

Relevance A commonly cultivated and demanded type of vegetable crops – cabbage Brassica oleracea L. – in the process of evolution and domestication was divided into three clusters: leafy cabbage, headed cabbage and broccoli (cauliflower). According to modern data, cabbage originated in culture from the large-leaved forms of leafy cabbage. Portuguese tronchuda is a transitional form to a typical headed cabbage. It is represented by leafy and semi-capped forms and evolutionarily related to Portuguese halega (Galician) leafy cabbage. White cabbage (Brassica oleracea L. convar. capitata (DC.) Alef. var. capitata L. f. alba DC.) is the most significant in the world and Russian economy. The characteristic of cultures of Brassica oleracea L. (varieties of white cabbage and tronchuda) is given according to the most important biochemical quality characteristics. Methods The research material included 48 samples of cabbage, including 27 samples of 13 European cultivar types, including Dutch, Central European and South European cultivar types, 8 samples – two Russian northwestern and central Russian cultivar types and 13 samples – five oriental cultivar types of Caucasian and Turkish-Balkan origin, 9 samples – Portuguese cabbage tronchuda from the VIR collection, reflecting the ecological, geographic and genetic diversity of cabbage. Samples of cabbage were grown at the research and production base "Pushkin and Pavlovsk laboratories VIR" (St. Petersburg), biochemical analysis was carried out in the laboratory of biochemistry and molecular biology of VIR. Results As a result of using of the modern non-specific metabolic approach to the study of the biochemical composition in samples of white cabbage, about 100 components have been identified from the groups of organic acids, free amino acids, including essential, fatty acids, including essential, polyhydric alcohols, sugars, and also phenolic compounds, wax, nucleosides, and others. Regularity in the accumulation of nutrient and biologically active substances by cultures of Brassica oleracea L., by cultivar types and individual samples were revealed. As a result of our research using modern techniques, including the nonspecific metabolic approach, new data on the biochemical composition of white and semi-capped forms were obtained. The cabbage within the studied botanical varieties and groups of cultivar types has a complex biochemical composition that characterizes cabbage samples as samples with potentially high value (the role and importance of far from all compounds in the organization of healthy human nutrition is known), which confirms the need for in-depth control of the biochemical composition of plants when breeding new varieties. Samples with the optimal component composition for balanced human nutrition, which are proposed to be used in breeding for quality, including obtaining varieties for healthy (functional) and therapeutic and prophylactic nutrition of the population of theRussian Federation were found.