Abstract
The Metabolism of Neoplastic Tissues: Further Studies on the Hexosemonophosphate Oxidative Pathway
Highlights
If G-1-C14 serves as substrate, the HMP shunt contributes the unlabelled half of the glucose molecule to the lactate pool, whereas the labelled part of the glucose enters the lactate pool in the case of G-6-C14
The specific activities of the lactates derived from the two labelled glucoses may serve for the calculation of the relative contribution of the HMP shunt and E-M pathway to the conversion of glucose to lactate
Tissue slices from mouse and rat tumours were incubated in the presence of glucose-l- and -6-C14 and the C14 contents of the respiratory carbon dioxide, lactate, long-chain fatty acids, cholesterol, proteins and some protein-bound amino acids were measured
Summary
Unless stated otherwise transplanted mouse tumours were used in the present experiments. The incubation procedure and the isolation of the respiratory carbon dioxide, proteins, long-chain fatty acids, cholesterol and lactate, including the chemical degradation of lactate, has been described previously (Emmelot and Bosch, 195.5; van Vals and Emmelot, 1957). Glycine and alanine were separated by paper chromatography on Whatman 3 MM paper (Arnstein and Stankovic, 1956). The purity of the four amino acids was checked by paper chromatography on Whatman No 1 paper. Amino acids and lactate were combusted to carbon dioxide and measured as BaCO3 at " infinite " thickness. Fatty acids and cholesterol digitonide were plated directly and their radioactivity was measured at " infinite " thickness, and, if necessary, correction being made for self-absorption. The labelled glucoses were diluted with inactive glucose to give the desired isotope concentration
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