Abstract
The Metabolism of Neoplastic Tissues: Demonstration of Intermediates and Reaction Sequences of the Hexose Monophosphate Oxidative Pathway
Highlights
The results indicated that the hexose monophosphate (HMP)* oxidative route might be operative
Experiments were undertaken to investigate whether the enzymes involved in the HMP oxidative pathway could be demonstrated in extracts prepared from tumours which had been studied with the tracer technique
By the use of cell-free extracts, homogenates and slices of transplanted tumours, enzymes, intermediates and reactions participating in the hexose monophosphate oxidative pathway were demonstrated
Summary
The G6-P and 6-PG dehydrogenases were determined according to Glock and McLean (1953, 1954). Results are given in units of enzyme activity per gram fresh weight of tissue, one unit being defined as the quantity of enzyme which at 20°C and pH 7-4 reduces 0-01 It. mole TPN per min. Optical densities were read at 340 m/t. In some cases the reduced TPN was measured by a coupled oxidation-reduction involving glutathione (Anderson et al 1952; Rall and Lehninger, 1952; Kinoshita and Masurat, 1954). Reduced glutathione was determined according to the nitroprusside method of Grunert and Philips (1951). In other experiments 2: 6dichlorophenol-indophenol was used as electron acceptor (Axelrod et al, 1953)
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