The mechanism of rabbit liver mitochondrial oxidative damage about obstructive jaundice

Abstract

Objective To explore the mechanism of rabbit liver mitochondrial oxidative damage about obstructive jaundice.Methods The 30 New Zealand rabbits were divided into two groups,which were the control group and the obstructive jaundice group.After the rabbits were modeled for 7 days,they were taken in liver tissue 100 mg,which were detected the index——8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA).Results (1) The results of fluorescence immunohistochemistry:the liver cells of rabbits in the obstructive jaundice group (pre-treatment) were found to have significant mitochondrial deoxyribonucleicacid (mtDNA) oxidative damage.(2) Levels of 8-OHdG in the liver mitochondria:the 8-OHdG levels of the control group and the obstructive jaundice group were (210 ± 78) pg/mg protein and (1920 ±231) pg/mg protein,There is significant difference between them (P ＜0.01).(3) Levels of MDA in the liver mitochondria:the MDA levels of the control group and the obstructive jaundice group were (0.21 ± 0.09) nmol/mg protein and (1.08 ± 0.25) nmol/mg protein,There is significant difference between them (P ＜ 0.01).Conclusion The obstructive jaundice of rabbits can cause hepatic mitochondrial oxidative stress injury,which mechanism maybe remove 8-OHdG and MDA in the liver mitochondria. Key words: Obstructive jaundice; Liver cells; Mitochondria; Pxidative damage; Malondialdehyde ; 8-hydroxydeoxyguanosine